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KMID : 0545119940040040274
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 4 p.274 ~ p.277
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Cell Cycle Regulated Expression of Subcloned Chicken H3 Histone Genes and Their 5´ Flanking Sequences
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Son Seung-Yeol
Tae Gun-Sik
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Abstract
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We subcloned two chicken H3 histone genes and transfected them into Rat 3 cell line. One contains 300 by 5´ to its cap site and the other contains 130 by 5´ to its cap site when cloned into plasmids. Both of them showed S phase specific expression of their mRNA about 8 fold higher (during S phase) than during G1 phase. This means that only 130 by 5´ to its cap site was enough to confer cell cycle regulated expression of the latter gene. The DNA sequences of their 5´ flanking region did not reveal any particular homologies or subtype-specific sequences. The DNA sequence data also showed that even though the protein coding regions of the histone genes have been conserved exceptionally well throughout evolution, their 5´ untranslated regions have not been conserved as well.
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